Real Phsc Please Stand Up! [editorial] What Defines a Pluripotent Hematopoietic Stem Cell (phsc): Will The
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منابع مشابه
In vivo administration of stem cell factor to mice increases the absolute number of pluripotent hematopoietic stem cells.
We have examined the effects of administration of stem cell-factor (SCF) on the number and distribution of pluripotent hematopoietic stem cells (PHSC) in normal mice. Using the competitive repopulation assay we found that in vivo administration of SCF increases the absolute number of PHSC per mouse threefold. The increased numbers of PHSC are found in the peripheral blood and spleen of the SCF-...
متن کاملEfficient retrovirus transduction of mouse pluripotent hematopoietic stem cells mobilized into the peripheral blood by treatment with granulocyte colony-stimulating factor and stem cell factor.
Cytokine-mobilized peripheral blood cells have been shown to participate in hematopoietic recovery after bone marrow (BM) transplantation, and are proposed to be useful targets for retrovirus-mediated gene transfer protocols. We treated mice with granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) to mobilize hematopoietic progenitor cells into the peripheral blood. These c...
متن کاملPurification and characterization of heterogeneous pluripotent hematopoietic stem cell populations expressing high levels of c-kit receptor.
Mouse pluripotent hematopoietic stem cells (PHSC) were fractionated based on size and density using counterflow centrifugal elutriation (CCE). These heterogeneous PHSC populations were further enriched by subtraction of cells with lineage-specific markers (Lin-) followed by positive sorting for c-kit expression. The cells were characterized for their functional and biochemical properties. We de...
متن کاملSplenic primitive hematopoietic stem cell (PHSC) activity is enhanced by steel factor because of PHSC proliferation.
To test whether primitive hematopoietic stem cells (PHSCs) are stimulated by Steel (SI) factor (c-kit ligand) in vivo, donor mice were studied after three or seven daily injections of SI factor. PHSC activity was measured as long-term erythroid and lymphoid competitive repopulating ability. Cells to be tested (usually marrow or spleen cells from treated donors) were mixed with untreated competi...
متن کاملLimited proliferative potential of primitive hematopoietic stem cells: hematopoiesis by clonal succession.
The hierarchy of hematopoietic stem cells (HSC) is represented by several categories of maturating pluripotent progenitors. Most of them are the members of transitional cell populations and, obviously, have no capacity for self-maintenance, i. e., are not capable of giving rise to self-replicating offspring with the same proliferative potential as the parent had [1]. The foundator of this hiera...
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تاریخ انتشار 1994